QuantiTect SYBR(R) Green RT-PCR Kit. For one-step qRT-PCR using SYBR Green I for gene expression analysis. Kit contents: Qiagen QuantiTect SYBR Green RT-PCR Kit, 1000 x 50L rxns, SYBR Green I, RNA Sample, Real-time One-step RT-PCR Reaction, Ideal for Real-time Quantification of RNA Targets, For One-step qRT-PCR Using SYBR Green I for Gene Expression Analysis, Includes 25mL 2x Qiagen QuantiTect SYBR Green RT-PCR Master Mix, 0.5mL Qiagen QuantiTect RT Mix, 20mL RNase-free Water. Benefits: High specificity in one-step RT-PCR. Reliable quantification of low-abundance transcripts. Accurate quantification over several logs of template. No need to optimize reaction and cycling conditions