EndoFree Plasmid Kits. For purification of up to 10 mg endotoxin-free advanced transfection-grade plasmid or cosmid DNA. Kit contents: Qiagen EndoFree Plasmid Mega Kit, 500mL to 2.5L Culture Volume, Anion-exchange Technology, Transfection-grade Plasmid or Cosmid DNA Purification, Manual Processing, 220 min. Time/Run, <2.5mg Yield, Transfection, High Yields of High-copy Plasmid DNA, For Purification of up to 2.5mg Endotoxin-free Advanced Transfection-grade Plasmid or Cosmid DNA, Ideal for Gene Therapy Application, Includes 5 Qiagen-tip 2500, Reagents, 5 QIAfilter Mega-Giga Cartridges, Endotoxin-free Buffers. Benefits: Less than 0.1 EU/micro g DNA achieved. Fast procedure, with integrated endotoxin removal. High yields of high-copy plasmid DNA. LyseBlue for optimum lysis and maximum DNA yield