Supplier:
New England Biolabs, Inc.EpiMark® 5-hmC and 5-mC Analysis Kit
5-methylcytosine (5-mC) is the predominant epigenetic mark in mammalian genomic DNA. 5-hydroxymethylcytosine (5-hmC) is a newly discovered epigenetic modification that is presumably generated by oxidation of 5-mC by the TET family of cytosine oxygenases (1,2).
Techniques exist that can identify 5-mC in genomic DNA, but the most commonly used method, bisulfite sequencing, is laborious and cannot distinguish between 5-mC from 5-hmC (3).
The EpiMark® 5-hmC and 5-mC Analysis Kit can be used to analyze and quantitate 5-methylcytosine and 5-hydroxymethylcytosine within a specific locus.
The kit distinguishes 5-mC from 5-hmC by the addition of glucose to the hydroxyl group of 5-hmC via an enzymatic reaction utilizing T4 β-glucosyltransferase (T4-BGT). When 5-hmC occurs in the context of CCGG, this modification converts a cleavable MspI site to a noncleavable one.
Prices direct from New England Biolabs, Inc.
Quick response times
Exclusive Labsave savings/discounts
Latest promotions
BaySpec, Inc. is offering special academic discount with the new NomadicTM multi-excitation confocal Raman microscope. NomadicTM is the only Raman microscope...
15% off on BMT Incubators - Climacell, Friocell, Incucell / Incucell V. On-Line Special 15% Discount on all Incubators, including Incubator options. •...
Order any Aries FilterWorks High Purity Lab Water Purification System an receive the first Years’ worth of Carbon and/or DI filters free! Please...
Combination Really-Flow™ Fluoride electrode, Epoxy body, Single Junction, Refillable with 1 meter low-noise cable and BNC connector. Weiss...
Avoid the risks associated with sample transfer and reduce hands-on time when you bind, wash, elute and/or concentrate your protein in the all-in-one...
Use promo code EASY3 to receive 13% off and FREE shipping!Experience a new dimension of electronic pipetting with the NEW Easypet 3 pipette controller. The...
All Randox Polyclonal and Monoclonal antibodies along with our extensive range of Human recombinant proteins are being offered online with a 10% discount...
Jenway’s 73 series spectrophotometer range provides four models with a narrow spectral bandwidth of 5nm and an absorbance range of –0.3 to 2.5A,...
Top suppliers
NuAire, Inc.
63 products
Qiagen
306 products
Eppendorf
1 products
Randox Laboratories
190 products
Panasonic Healthcare Company
5 products
Life Technologies
1 products
Nikon Instruments Europe
11 products
Olympus Europa Holding GmbH
3 products
GE Healthcare Life Sciences
2 products
Tecan Trading AG
19 products
BD (Becton, Dickinson and Company)
KEYENCE Corporation
5 products
RANDOX TOXICOLOGY
5 products
Randox Food Diagnostics
6 products