HotStarTaq Plus Master Mix Kit. For fast and highly specific amplification in all applications. Kit contents: Qiagen HotStarTaq Plus Master Mix Kit, 2500 x 20micro L rxns, 5U/L, Genomic DNA and cDNA Sample, 10 min. at 97C. 60 min. at 94C Half-life, 2 to 4 kb/min. at 72C Extension Rate, PCR Amplification Reaction Type, Ideal for PCR, RT-PCR, Includes 25mL HotStarTaq Plus Master Mix (Contains 2500U of HotStarTaq Plus DNA Polymerase total, PCR Buffer with 3mM MgCl2 and 400micro M of Each dNTP), 5.5mL CoralLoad Concentrate, 2 x 20mL RNase-free Water. Benefits: Fast 5-minute enzyme activation time. Fewer pipetting steps reduces the risk of contamination. High PCR specificity without the need for optimization. Optional ready-to-load buffer additive for easier handling