QIAGEN Plasmid Kits. For purification of up to 10 mg transfection-grade plasmid or cosmid DNA. Kit contents: Qiagen Plasmid Midi Kit, 25 preps, 25 to 100mL Culture Volume, Manual (Centrifugation) Processing, 150 min. Time/Run, Anion-exchange Technology, 1 Sample/Run, For Purification of up to 100g Transfection-grade Plasmid or Cosmid DNA, Ideal for Cloning, Sequencing, Capillary Sequencing, In vitro Transcription, Includes 25 Qiagen to tip 100, Reagents, Buffers. Benefits: Purity equivalent to 2 x CsCl gradient centrifugation. High yields of plasmid DNA. Cost-effective preparations. LyseBlue for optimum lysis and maximum DNA yield