QIAGEN Plasmid Plus Kits. For the fastest, most convenient purification of up to 10 mg transfection-grade plasmid DNA. Kit contents: Qiagen Plasmid Plus Giga Kit, 5 preps, 2500mL Culture Volume, Transfection-grade Plasmid or Cosmid DNA Purification, Advanced Silica Technology, Manual Processing, 50 min. Time/Run, Up to 10mg Yield, Ideal for Transfection of Most Cell Lines, Cloning, Enzymatic Restriction, In-vitro Translation/Transcription, Sequencing, Includes 5 Qiagen Plasmid Plus Mega Columns, Extender Tubes, Reagents, Buffers, 5 QIAfilter Mega-Giga Cartridges. Benefits: Rapid purification of up to 24 samples . Fast vacuum-based parallel processing . Low elution volumes for highly concentrated DNA . Very low endotoxin levels (<1 EU/micro g) due to dedicated wash buffer