QIAGEN Plasmid Plus Kits. For the fastest, most convenient purification of up to 10 mg transfection-grade plasmid DNA. Kit contents: Qiagen Plasmid Plus Midi Kit, 25 preps, 25 to 50mL Culture Volume, Transfection-grade Plasmid or Cosmid DNA Purification, Advanced Silica Technology, Manual Processing, 20 min. Time/Run, Up to 1mg Yield, Ideal Transfection of most Cell Lines, Cloning, Enzymatic Restriction, For the Fastest, Most Convenient Purification of up to 250micro g Transfection-grade Plasmid DNA, Includes 25 Qiagen Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 25 QIAfilter Midi Cartridges. Benefits: Rapid purification of up to 24 samples . Fast vacuum-based parallel processing . Low elution volumes for highly concentrated DNA . Very low endotoxin levels (<1 EU/micro g) due to dedicated wash buffer