QIAGEN Plasmid Kits. For purification of up to 10 mg transfection-grade plasmid or cosmid DNA. Kit contents: Qiagen-tip 500, 25 Columns, 100 to 500mL Culture Volume, 160 min. Time/Run, 1 Sample/Run, <500g Yield, Manual Processing, Anion-exchange Technology, Ideal for Cloning, Sequencing, Capillary Sequencing etc. , For Gravity-flow Purification of up to 500g Transfection-grade Plasmid DNA. Benefits: Purity equivalent to 2 x CsCl gradient centrifugation. High yields of plasmid DNA. Cost-effective preparations. LyseBlue for optimum lysis and maximum DNA yield